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61.
采用二甲基亚砜(DMSO)作为冻干标准物质的溶剂,以重量法准确配制维生素K_1/DMSO溶液,再进行分装、冷冻干燥,经定性分析、定值分析、均匀性检验、稳定性考察和不确定度评定,研制了维生素K_1冻干标准物质。以维生素K_1纯度标准物质溶液为校准品,对得到的冻干物质进行了高效液相色谱法定值分析,冻干标准物质的准确定值结果为0.96 mg/mL,相对扩展不确定度为7%。建立的维生素K_1冻干标准物质研制方法,对于临床检验中维生素K_1的准确测定和相关疾病的正确诊断治疗以及维生素K_1长期保存具有重要意义。 相似文献
62.
目的: 探讨双PI3K/mTOR 抑制剂 NVPBEZ235对宫颈癌Hela细胞的放射增敏作用。方法: 四甲基偶氮唑盐(MTT)及集落克隆法测定各因素对Hela细胞的抑制作用。通过流式细胞仪检测药物联合射线(irradiation,IR)对宫颈癌细胞周期的影响。结果: 与对照组相比,NVPBEZ235在体外能够抑制人宫颈癌细胞的生长。NVPBEZ235、IR联合作用细胞,在体外可以使细胞周期阻滞于G2/M期,二者具有协同作用,联合作用效果显著大于单药作用。结论: 20% IC50 NVPBEZ235在体外能够促进细胞对射线照射的敏感性,抑制细胞的增殖从而实现对宫颈癌细胞的放射增敏作用。 相似文献
63.
Interleukin-22 (IL-22) is a member of IL-10 cytokine family which is expressed in activated T cells
predominantly and in activated natural killer cells at lower levels. Previous studies have demonstrated the link between
elevated levels of IL-22 and disease severity of psoriasis, Crohn’s disease, rheumatoid arthritis and interstitial lung
diseases. However, the function of IL-22 in the development and progression of colorectal cancer (CRC) remains
elusive. In this study, we first evaluated the IL-22/IL-22R1 level in CRC patients, and found that tumor tissues
have more active expression of IL-22 and IL-22R1 than normal tissues, presenting correlation with the degree of
differentiation of tumor tissues. Subsequently, Caspase and cell viability assays were performed on SW-480 cell line
which expresses high level of IL-22R1 to examine if the supplementation of IL-22 has an impact on apoptosis and
proliferation. In comparison with treatment of 5-FU, supplementation of IL-22 promoted cell proliferation and
ameliorated apoptosis. To unveil signal transduction upon activation of IL-22R, we examined the phosphorylation
of STAT3 in SW-480 cell line following supplementation of IL-22. The treatment of IL-22 also increased the level of
p-Akt, an essential component in PI3K/Akt pathway. Although the link between STAT3 phosphorylation and PI3K/
Akt activation remains to be explored, our study revealed the mechanism underlying the effects of IL-22R activation on
apoptosis as well as tumor differentiation, indicating the prognostic value of IL-22/IL-22R. 相似文献
64.
软测量建模能够有效地解决生产过程中在线分析仪表测量滞后大、价格昂贵、维护保养复杂等问题。目前,基于数据驱动的神经网络是软测量建模的主要工具之一。而在建模数据的采集过程中,主导变量的采集相对辅助变量要困难得多,由此产生了大量缺失标签的数据。但传统的软测量建模方法却忽视了这些无标签数据,只利用少量的有标签数据建模,从而影响了模型的预测精度。为了解决标签缺失的问题,采用最近邻算法对无标签数据进行伪标记,同时设计了由卷积操作与门限循环单元神经网络(GRU)结合的网络结构来进一步利用无标签数据,提取不同时刻数据中的动态特征,提高神经网络的预测精度。最后将该方法应用于丙烯精馏塔塔顶丙烷浓度的预测,实验结果表明该模型能有效处理非线性动态系统的标签缺失问题,具有更高的预测精度。 相似文献
65.
One of the technical bottlenecks of traditional laser-induced breakdown spectroscopy (LIBS) is the difficulty in quantitative detection caused by the matrix effect. To troubleshoot this problem, this paper investigated a combination of time-resolved LIBS and convolutional neural networks (CNNs) to improve K determination in soil. The time-resolved LIBS contained the information of both wavelength and time dimension. The spectra of wavelength dimension showed the characteristic emission lines of elements, and those of time dimension presented the plasma decay trend. The one-dimensional data of LIBS intensity from the emission line at 766.49 nm were extracted and correlated with the K concentration, showing a poor correlation of R2c=0.0967, which is caused by the matrix effect of heterogeneous soil. For the wavelength dimension, the two-dimensional data of traditional integrated LIBS were extracted and analyzed by an artificial neural network (ANN), showing R2v=0.6318 and the root mean square error of validation (RMSEV)=0.6234. For the time dimension, the two-dimensional data of time-decay LIBS were extracted and analyzed by ANN, showing R2v=0.7366 and RMSEV=0.7855. These higher determination coefficients reveal that both the non-K emission lines of wavelength dimension and the spectral decay of time dimension could assist in quantitative detection of K. However, due to limited calibration samples, the two-dimensional models presented over-fitting. The three-dimensional data of time-resolved LIBS were analyzed by CNNs, which extracted and integrated the information of both the wavelength and time dimension, showing the R2v=0.9968 and RMSEV=0.0785. CNN analysis of time-resolved LIBS is capable of improving the determination of K in soil. 相似文献
66.
Todd E. Katzner Philip J. Turk Adam E. Duerr Tricia A. Miller Michael J. Lanzone Jeff L. Cooper David Brandes Junior A. Tremblay Jér?me Lema?tre 《Journal of the Royal Society Interface》2015,12(112)
Large birds regularly use updrafts to subsidize flight. Although most research on soaring bird flight has focused on use of thermal updrafts, there is evidence suggesting that many species are likely to use multiple modes of subsidy. We tested the degree to which a large soaring species uses multiple modes of subsidy to provide insights into the decision-making that underlies flight behaviour. We statistically classified more than 22 000 global positioning satellite–global system for mobile communications telemetry points collected at 30-s intervals to identify the type of subsidized flight used by 32 migrating golden eagles during spring in eastern North America. Eagles used subsidized flight on 87% of their journey. They spent 41.9% ± 1.5 (, range: 18–56%) of their subsidized northbound migration using thermal soaring, 45.2% ± 2.1 (12–65%) of time gliding between thermals, and 12.9% ± 2.2 (1–55%) of time using orographic updrafts. Golden eagles responded to the variable local-scale meteorological events they encountered by switching flight behaviour to take advantage of multiple modes of subsidy. Orographic soaring occurred more frequently in morning and evening, earlier in the migration season, and when crosswinds and tail winds were greatest. Switching between flight modes allowed migration for relatively longer periods each day and frequent switching behaviour has implications for a better understanding of avian flight behaviour and of the evolution of use of subsidy in flight. 相似文献
67.
Mircea V. Diudea 《Fullerenes, Nanotubes and Carbon Nanostructures》2019,27(4):367-374
Rhombellanes are mathematical structures, proposed by us in 2017; they may appear both in periodic crystals or in finite structures. The simplest rhombellane is rbl.5 or K2.3, the complete bipartite graph. In this paper, rhombellation operation is iteratively applied on three classes of structures: cube, tori and cubic pcu-network, respectively. The structural topological parameters are detailed. 相似文献
68.
One of the most accurate types of prototype selection algorithms, preprocessing techniques that select a subset of instances from the data before applying nearest neighbor classification to it, are evolutionary approaches. These algorithms result in very high accuracy and reduction rates, but unfortunately come at a substantial computational cost. In this paper, we introduce a framework that allows to efficiently use the intermediary results of the prototype selection algorithms to further increase their accuracy performance. Instead of only using the fittest prototype subset generated by the evolutionary algorithm, we use multiple prototype subsets in an ensemble setting. Secondly, in order to classify a test instance, we only use prototype subsets that accurately classify training instances in the neighborhood of that test instance. In an experimental evaluation, we apply our new framework to four state-of-the-art prototype selection algorithms and show that, by using our framework, more accurate results are obtained after less evaluations of the prototype selection method. We also present a case study with a prototype generation algorithm, showing that our framework is easily extended to other preprocessing paradigms as well. 相似文献
69.
Fang Wang Li-dan Deng Ze-wei Wu Kai Ji Qiao Chen Xing-mao Jiang 《International Journal of Hydrogen Energy》2021,46(40):20815-20824
Ru nanoparticles supported on SiO2 microspheres (Ru/SiO2-GUS) were prepared by the glucose-urea-metallic salt method and applied in the decomposition of ammonia. In the glucose-urea-metallic salt method, glucose as the carbon template plays a significant role in the formation of diffusion-beneficial structural properties of Ru/SiO2-GUS, and also induceds the modification of the electronic state of Ru. Ru/SiO2-GUS exhibited higher catalytic activity compared with the catalyst prepared with the impregnation method. The catalytic performance of Ru/SiO2-GUS was further enhanced with the addition of either K or Cs——the addition order and amount strongly affecting the catalytic performance. When the ratio of K/Cs to Ru is 2, the alkali metal (KOH/CsOH) solution is added in the homogeneous solution of glucose, urea, RuCl3 and the colloidal silica, the promotion effect of K/Cs is the strongest, particularly under lower reaction temperatures. However, the promotion effects of K and Cs are different as reveled by the combined results of H2-TPR, XPS and NH3-TPSR. More NH3 can be absorbed on K–Ru/SiO2-GUS and the electron density of Ru decreased. By contrast, more metallic Ru formed on Cs–Ru/SiO2-GUS, facilitating N2 recombination. 相似文献
70.
在射线内透偏心法透照中,采用一次贴片,多次曝光,分次取片的检测方法效率高,但是检测人员容易忽视透照厚度比K值的变化,使得K值不符合标准要求。本文通过计算与分析,提出在内透偏心法透照中应首先确定一次最大偏心距离Smax,再通过和实际偏心距离对比,确定曝光方式,并提出在偏心透照方法中为保证底片的黑度和灵敏度应注意的一些问题。 相似文献